Product Description
TCRab/Vb2 Antibody [TCR-3] (FITC) | 99-228 | ProSci
Host: Mouse
Reactivity: Chicken, Peacock, Guinea Fowl
Homology: N/A
Immunogen: CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
Research Area: Immunology
Tested Application: E, ICC, Flow, Blocking, IP, Adhesion, Complement Mediated Cell Depletion
Application: TCRab/Vb2 Antibody [TCR-3] for use in flow cytometry and immunohistochemistry assays.
Specificiy: TCRalphabeta/Vbeta2
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: [TCR-3]
Isotype: IgG1
Conjugate: FITC
Physical State: Liquid
Buffer: Supplied in PBS/NaN3
Concentration: 0.5 mg/mL
Storage Condition: Store vial at 2-8˚C
Alternate Name: T3/TCR complex, TCR alpha/beta
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of alphabeta T cells appear in the thymus subsequent to the appearance of gammadelta T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- subpopulations. The TCR-3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR-3+] cells in the blood and spleen express relatively high levels of the TCR-3/CD3 receptor complex and are “single positive” with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (~ 80% CD4+ vs ~ 20% CD8+) .