Product Description
TCRgd Antibody [TCR-1] | 99-219 | ProSci
Host: Mouse
Reactivity: Chicken, Milk Snake
Homology: N/A
Immunogen: Outbred chicken thymocytes and Ig-negative blood lymphocytes
Research Area: Cell Cycle
Tested Application: E, Flow, Blocking, IP, Complement Mediated Cell Depletion
Application: TCRgd Antibody [TCR-1] for use in flow cytometry, immunohistochemistry, immunoprecipitation, and depletion assays.
Specificiy: TCRgammadelta
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: [TCR-1]
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration: 0.5 mg/mL
Storage Condition: Store vial at 2-8˚C
Alternate Name: T3/TCR complex, TCR gamma/delta
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike human and mouse in which gammadelta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gammadelta T cells. The frequency of TCRgammadelta cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRgammadelta+ cells in the thymus and blood are CD4-CD8- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgammadelta+ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of gammadelta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gammadelta T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.