Product Description
TGF-alpha Antibody [SPM542] | 34-170 | ProSci
Host: Mouse
Reactivity: Human, Rabbit, Zebrafish
Homology: N/A
Immunogen: Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.
Research Area: Growth Factors, Antibody Pairs, Functional Assays
Tested Application: Flow, IF, IHC
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE) : 2-4 ug/ml for 30 min at RT (1)
The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: SPM542
Isotype: IgM, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.