Product Description
TIMP2 Antibody [SPM356] | 33-734 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: An amino acid sequence from the N-terminal region of the human protein was used as the immunogen for this TIMP2 antibody.
Research Area: Signal Transduction
Tested Application: IHC-P, IF, WB
Application: IHC (FFPE) : 0.5-1 ug/ml for 30 min at RT
WB: 0.5-1 ug/ml
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: SPM356
Isotype: IgG2a, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: TIMP2, Metalloproteinase inhibitor 2, TIMP-2, CSC-21K
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2) . It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B) . TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.