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Product Description
PLKK (phospho Ser482 / Ser486 / Ser490) Antibody | XPS-1022 | ProSci
Host: Rabbit
Reactivity: Frog
Homology: N/A
Immunogen: PLKK (Ser482/486/490) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus Phospho-Ser482, 486, 490 PLKK.
Research Area: Signal Transduction, Phospho-Specific
Tested Application: WB
Application: Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. Xenopus is the only species tested to date. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificiy: PLKK antibody is specific for PLKK phosphorylated at Ser482, 486, 490.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 120
Validation: N/A
Isoform: N/A
Purification: Affinity Purified
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: N/A
Concentration: N/A
Storage Condition: For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490) . Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482, 486, 490 in PLKK.
