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Product Description
CD3e Antibody | 33-082 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat, Primate, Guinea Pig, Hamster, Horse, Mole Rat, Pig, Rabbit, Sheep, Cat, Chicken, Bovine, Dog, Frog, Opossum, Elephant
Homology: N/A
Immunogen: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
Research Area: Cancer, Immunology, Signal Transduction
Tested Application: WB, Flow, IP
Application: Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cells
WB: 0.5-1.0 ug/ml
IP: 1-2ug/500ug protein lysate
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein A purified CD3e antibody
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes the epsilon-chain of CD3 (CD3e) , which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
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