Product Description
Chondroadherin Antibody | 8421 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen: Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin.
The immunogen is located within the last 50 amino acids of Chondroadherin.
Research Area: Stem Cell, Homeostasis
Tested Application: E, WB, ICC
Application: Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Specificiy: Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.
Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2: Cat. No. 17-201 - 3T3/BALB Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 38 kDa
Observed: 36 kDa
Validation: N/A
Isoform: N/A
Purification: Chondroadherin antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Chondroadherin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Chondroadherin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Cartilage leucine-rich protein, CHAD, SLRR4A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1, 2) . It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4) . Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2, 4, 5) .