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Product Description
Hrk Antibody | 3771 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk.
The immunogen is located within the last 50 amino acids of Hrk.
Research Area: Signal Transduction, Neuroscience
Tested Application: E, WB
Application: Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1414 - Mouse Pancreas Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 10 kDa
Observed: 12 kDa
Validation: N/A
Isoform: N/A
Purification: Hrk Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Hrk Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Hrk antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Hrk Antibody: DP5, HARAKIRI, BID3, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) -only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
