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Product Description
IL-1a Antibody | XP-5174 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-1alpha (human Interleukin-1-alpha) .
Research Area: Immunology, Chemokines & Cytokines, Antibody Pairs, Functional Assays
Tested Application: E, WB, Neut
Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1α (20 ng/mL) , a concentration of 1.0 - 2.0 μg/mL of this antibody is required.
ELISA:
To detect hIL-1α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1α.
Sandwich:
To detect hIL-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-1α (XP-5174Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1α.
Western Blot:
To detect hIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti-hIL-1alpha specific antibody was purified by affinity chromatography employing immobilized hIL-1alpha matrix.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: IL-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: IL1, IL-1A, IL1F1, IL1-ALPHA, Interleukin-1 alpha, Hematopoietin-1, IL-1 alpha
User Note: Centrifuge vial prior to opening.
BACKGROUND: N/A
