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Product Description
IL-7 Antibody | XP-5197 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-7 (human Interleukin-7) .
Research Area: Immunology, Chemokines & Cytokines, Functional Assays
Tested Application: E, WB, Neut
Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-7 (1.00 ng/mL) , a concentration of 0.016 0.03 μg/mL of this antibody is required.
ELISA:
To detect hIL-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-7.
Sandwich:
To detect hIL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-7 (XP-5197Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-7.
Western Blot:
To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti-hIL-7 specific antibody was purified by affinity chromatography employing immobilized hIL-7 matrix.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: IL-7 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: IL-7Interleukin-7IL-7
User Note: Centrifuge vial prior to opening.
BACKGROUND: Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA) . IL-7 stimulates the proliferation of CD4+/CD8+ cells.
![IL-7 Antibody [NYR-hi7]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/555195/764067/porsci_lo__79508.1648973713__30560.1649086593.png?c=2 "IL-7 Antibody [NYR-hi7]")
