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Product Description
MARCH8 Antibody | 4885 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (76%)
Immunogen: MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8.
The immunogen is located within the last 50 amino acids of MARCH8.
Research Area: Innate Immunity
Tested Application: E, WB, ICC
Application: MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: MARCH8 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: MARCH8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: MARCH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: MARCH8 Antibody: MIR, c-MIR, RNF178, MARCH-VIII, MIR, E3 ubiquitin-protein ligase MARCH8, Cellular modulator of immune recognition
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain) . MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.
