Product Description
ENO1 Antibody, KO Validated | 13-583 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1) .
Research Area: Cancer, Signal Transduction, Transcription
Tested Application: WB, IF, IP
Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: PC-3
Positive Control 2: Ramos
Positive Control 3: NCI-H460
Positive Control 4: HeLa
Positive Control 5: THP-1
Positive Control 6: Mouse kidney
Molecular Weight: Observed: 47kDa
Validation: Antibody is Knockout validated.
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: ENO1, NNE, PPH, MPB1, ENO1L1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.