Product Description
EIF5B Antibody | 19-922 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 901-1220 of human EIF5B (NP_056988.3) .
Research Area: Other
Tested Application: IF
Application: IF: 1:10 - 1:100
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: IF2, eukaryotic translation initiation factor 5B, eIF-5B, translation initiation factor IF-2, translation initiation factor IF2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.