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![APRIL Antibody [Sacha-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/552822/760398/porsci_lo__79508.1648973713__80786.1649086078.png?c=2 "APRIL Antibody [Sacha-2]")
![APRIL Antibody [Sacha-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/552822/760398/porsci_lo__79508.1648973713__80786.1649086078.png?c=2 "APRIL Antibody [Sacha-2]")
Product Description
APRIL Antibody [Sacha-2] | XA-1025 | ProSci
Host: Rat
Reactivity: Human, Mouse
Homology: N/A
Immunogen: APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250) .
Research Area: Chemokines & Cytokines, Cancer
Tested Application: E, ICC, Flow
Application: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture) , Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μm sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μg/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line) . Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 μg/mL of Sacha-2 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.
Clonality: Monoclonal
Clone: Sacha-2
Isotype: IgG2a
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS containing 0.02% sodium azide
Concentration: N/A
Storage Condition: APRIL antibody can be stored at 4˚C, stable for one year.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: N/A
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