Product Description
Bmf Antibody [9G10] | 36-173 | ProSci
Host: Rat
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Recombinant mouse Bmf.
Research Area: Apoptosis, Cancer, Immunology
Tested Application: Flow, IP, WB
Application: Flow Cytometry: (overexpressed in cell lines) . Immunoprecipitation: (1-5ug/ml) . Western Blot: (1-2ug/ml) .
Specificiy: Recognizes human and mouse Bmf. Detects several cross-reactivity bands of unknown identity in mice by western blot.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: >95% (SDS-PAGE)
Clonality: Monoclonal
Clone: 9G10
Isotype: IgG2a, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. In PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.
Alternate Name: Bcl-2-modifying Factor
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3) , and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2) , which may be important for sensing intracellular damage and triggering apoptosis.