Product Description
DR6 Antibody [Luke-1] | XA-1015 | ProSci
Host: Rat
Reactivity: Human
Homology: N/A
Immunogen: DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6;Fc (aa. 42-335) .
Research Area: Apoptosis
Tested Application: Flow
Application: DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1) . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line) . Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: DR6 (monoclonal Luke-1) antibody was purified to ≥95% in SDS-PAGE.
Clonality: Monoclonal
Clone: Luke-1
Isotype: IgG2a
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS containing 0.02% sodium azide
Concentration: N/A
Storage Condition: DR6 antibody can be stored at 4˚C, stable for one year.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: N/A