Product Description
PARP-10 Antibody [5H11] | 36-172 | ProSci
Host: Rat
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.
Research Area: Apoptosis, Cancer, Innate Immunity, Obesity
Tested Application: ICC, IP, WB
Application: Immunocytochemistry: (1:50-1:200) . Immunoprecipitation: (1:10) . Western Blot: (1:50) . Optimal conditions must be determined individually for each application.
Specificiy: Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 5H11
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide.
Concentration: N/A
Storage Condition: Stable for at least 1 year after receipt when stored at +4˚C.
Alternate Name: ADP-ribosyltransferase Diphtheria Toxin-like 10; Poly (ADP-ribose) Polymerase-10
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: PARP-10. PARP-10 mono (ADP-ribosyl) ates (MARylates) NEMO (NF-kappaB essential modulator) , which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono (ADP-ribosyl) ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.