Product Description
Progranulin Antibody | 36-293 | ProSci
Host: Rat
Reactivity: Mouse
Homology: N/A
Immunogen: Recombinant mouse progranulin.
Research Area: Cancer, Innate Immunity, Obesity
Tested Application: E, WB
Application: ELISA: (direct and indirect: 1:2, 000-1:5, 000) . Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Specificiy: Recognizes mouse progranulin. Detects a band of ~90kDa by Western blot. Weakly cross-reacts with human progranulin.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Condition: Stable for at least 6 months after receipt when stored at -20˚C.
Alternate Name: Proepithelin; PEPI; PC Cell-derived Growth Factor
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD) . In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer‘s Disease (AD) and potentially for MCI (Mild Cognitive Impairment) . Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.