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Product Description
DBX1 Antibody | 6455 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (87%)
Immunogen: DBX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human DBX1.
The immunogen is located within amino acids 100 - 150 of DBX1.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: DBX1 antibody can be used for detection of DBX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: BX1 antibody is predicted to not cross-react with other DBX protein family members
Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: DBX1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: DBX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: DBX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: DBX1 Antibody: Homeobox protein DBX1, Developing brain homeobox protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: DBX1 Antibody: DBX1 is implicated in patterning the central nervous system during embryogenesis. DBX1 homeodomain transcription factor is expressed in progenitors which are critical in establishing cell fate allocation and cell diversity. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. DBX1-derived neurons are essential for the transgenic expression and generation of respiratory behavior both in vitro and in vivo.
