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Product Description
FOXA2 Antibody | 7257 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%) , Mouse: (94%)
Immunogen: FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 .
The immunogen is located within amino acids 280 - 330 of FOXA2.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family.
Positive Control 1: Cat. No. 1310 - Human Bladder Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 51 kDa
Observed: 54 kDa
Validation: N/A
Isoform: N/A
Purification: FOXA2 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: FOXA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: FOXA2 Antibody: HNF3B, TCF3B, HNF3B, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2, HNF-3-beta
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: FOXA2 Antibody: FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation.
