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Product Description
NELF Antibody | 7225 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF.
The immunogen is located within amino acids 230 - 280 of NELF.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 58 kDa
Validation: N/A
Isoform: N/A
Purification: NELF Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: NELF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: NELF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: NELF Antibody: HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: NELF Antibody: NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH) , a disorder resulting in impaired pubertal maturation and reproductive function.
