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Product Description
OLIG1 Antibody | 7741 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Rat: (83%) , Mouse: (78%)
Immunogen: OLIG1 antibody was raised against an 18 amino acid peptide near the amino terminus of human OLIG1.
The immunogen is located within amino acids 50 - 100 of OLIG1.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: OLIG1 antibody can be used for detection of OLIG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: OLIG1 antibody is human specific. It is predicted to not cross-react with other members of the OLIG family of proteins.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 30 kDa
Observed: 29 kDa
Validation: N/A
Isoform: N/A
Purification: OLIG1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: OLIG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: OLIG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: OLIG1 Antibody: BHLHB6, BHLHE21, BHLHB6, Oligodendrocyte transcription factor 1, Class B basic helix-loop-helix protein 6, Oligo1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1) . Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2) . OLIG1 is first expressed in the dorsal portion of the p3 progenitor domain of the ventral neural tube while OLIG2 is first observed in the ventral most p3 domain (2) . OLIG1 has been shown to be a SMAD cofactor involved in cell motility induced by transforming growth factor-beta (TGF-beta) (3) .
