Product Description
ZEB1 Antibody | 5825 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1.
The immunogen is located within amino acids 530 - 580 of ZEB1.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, ICC, IF
Application: ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Multiple isoforms of ZEB1 are known to exist.
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: Cat. No. 10-201 - Human Liver Tissue Slide
Positive Control 3: Cat. No. 17-001 - HeLa Cell Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 124 kDa
Observed: 130 kDa
Validation: N/A
Isoform: N/A
Purification: ZEB1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ZEB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ZEB1 Antibody: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, TCF-8
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ZEB1 Antibody: ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.