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Product Description
IPG-2 is a yellow-green fluorescent, intracellular potassium (K⁺) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K⁺ concentration. It is a small, synthetic fluorochrome which incorporates a K⁺-binding moiety. When K⁺ binds, the quenching is relieved and the fluorescence dramatically increases. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Cat. Nos. B3140, B3141; Kd = 50 mM) and lower affinity than IPG-4 (Cat. Nos. B3144, B3145; Kd = 7 mM). IPG-2 AM is the membrane permeable form of the potasssium indicator (Cat. No. B3143). Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. IPG-2 is compatible with a wide variety of detectors including fluorescent microscopes, plate readers, flow cytometers, and fluorescent indicator-doped solid-state sensors.
IPG-2 is a yellow-green fluorescent, intracellular potassium (K⁺) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K⁺ concentration. It is a small, synthetic fluorochrome which incorporates a K⁺-binding moiety. When K⁺ binds, the quenching is relieved and the fluorescence dramatically increases. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Cat. Nos. B3140, B3141; Kd = 50 mM) and lower affinity than IPG-4 (Cat. Nos. B3144, B3145; Kd = 7 mM). IPG-2 AM is the membrane permeable form of the potasssium indicator (Cat. No. B3143). Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. IPG-2 is compatible with a wide variety of detectors including fluorescent microscopes, plate readers, flow cytometers, and fluorescent indicator-doped solid-state sensors.
Biovision | B3142 | IPG-2 AM DataSheet
Alternate Name/Synonyms: Potassium (K⁺) indicator; IPG-2 membrane permeable; IPG-2 acetoxymethyl ester; Fluorescent Potassium indicator
Appearance: Red orange powder
Formulation:
CAS Number:
Structure Available?: TRUE
Peptide sequence:
Salt Form: FALSE
Molecular Formula:
Molecular Weight: 1128
Cell-Permeable?: TRUE
Purity: ≥ 90%
Solubilities: ~2 mg/ml in DMSO
Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Country of Origin: USA
Tag Line: A membrane permeable fluorescent potassium indicator
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