Product Description
O-Propargyl-puromycin (OPP) is cell-permeable alkyne analog of the protein synthesis inhibitor puromycin (Cat. No. 1860) that efficiently incorporates into newly translated proteins in complete methionine-containing media. The resulting truncated C-terminal alkyne labeled proteins can subsequently be detected via Cu(I)-catalyzed click chemistry reaction that links it to a fluorescently tagged azide molecule. Thus, OPP provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy, high-content imaging, or flow cytometry.
O-Propargyl-puromycin (OPP) is cell-permeable alkyne analog of the protein synthesis inhibitor puromycin (Cat. No. 1860) that efficiently incorporates into newly translated proteins in complete methionine-containing media. The resulting truncated C-terminal alkyne labeled proteins can subsequently be detected via Cu(I)-catalyzed click chemistry reaction that links it to a fluorescently tagged azide molecule. Thus, OPP provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy, high-content imaging, or flow cytometry.
Biovision | B2590 | O-Propargyl-puromycin DataSheet
Alternate Name/Synonyms: OPP, (2S)-2-amino-N-((2S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-(prop-2-yn-1-yloxy)phenyl)propanamide, OP-puro
Appearance: Off-white solid
Formulation:
CAS Number: 1416561-90-4
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: No
Molecular Formula: C₂₄H₂₉N₇O₅
Molecular Weight: 495.53
Cell-Permeable?: True
Purity: ≥95% by HPLC
Solubilities: >20 mg/ml in DMSO
Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Country of Origin: USA
Tag Line: A nascent protein marker suitable for imaging
MDL Number:
PubChem CID: 71576433
SMILES: CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OCC#C)N)O
InChi: InChI=1S/C24H29N7O5/c1-4-9-35-15-7-5-14(6-8-15)10-16(25)23(34)29-18-17(11-32)36-24(20(18)33)31-13-28-19-21(30(2)3)26-12-27-22(19)31/h1,5-8,12-13,16-18,20,24,32-33H,9-11,25H2,2-3H3,(H,29,34)/t16-,17+,18+,20+,24+/m0/s1
InChi Key: