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Product Description
Ready-to-use fluorometric substrate for AEVD-dependent caspases. Activity of AEVD-dependent caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate AEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. Cell Lysis Buffer (Cat. #1067-100, -400), 2X Reaction Buffer (Cat. #1068-20, -80), DTT (1201-1) and other reagents used for caspase assays are also available separately.
AEVD-AFC is a ready-to-use fluorometric substrate for caspase-10 and related caspases that recognize the amino acid sequence AEVD.
Biovision | 1113 | Caspase-10 Substrate AEVD-AFC DataSheet
Alternate Name/Synonyms: Ac-AEVD-AFC, Caspase-10 Substrate, Fluorogenic
Appearance: Liquid
Formulation: DMSO (1 mM)
CAS Number: N/A
Structure Available?: No
Peptide sequence: Ac-Ala-Glu-Val-Asp-AFC
Salt Form: No
Molecular Formula: C₂₉H₃₄F₃N₅O₁₁
Molecular Weight: 685.6
Cell-Permeable?: No
Purity: >98% by HPLC
Solubilities: N/A
Handling: Protect from light and moisture
Country of Origin: USA
Tag Line: A caspase-10 substrate
MDL Number: N/A
PubChem CID: 44134611
SMILES: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(C)NC(=O)C
InChi: InChI=1S/C29H34F3N5O11/c1-12(2)24(37-26(45)18(7-8-21(39)40)35-25(44)13(3)33-14(4)38)28(47)36-19(11-22(41)42)27(46)34-15-5-6-16-17(29(30,31)32)10-23(43)48-20(16)9-15/h5-6,9-10,12-13,18-19,24H,7-8,11H2,1-4H3, (H,33,38)(H,34,46)(H,35,44)(H,36,47)(H,37,45)(H,39,40)(H,41,42)
InChi Key: OGDZWRZGOFQIOD-UHFFFAOYSA-N
