Product Description
Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence ATAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate ATAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.
ATAD-AFC is a ready-to-use fluorometric caspase-12 substrate and caspases that recognize the amino acid sequence ATAD
Biovision | 1117 | Caspase-12 Substrate ATAD-AFC DataSheet
Alternate Name/Synonyms: Ac-ATAD-AFC, Caspase-12 Substrate, Fluorogenic
Appearance: Liquid
Formulation: DMSO (4 mM)
CAS Number: N/A
Structure Available?: No
Peptide sequence: Ac-Ala-Thr-Ala-Asp-AFC
Salt Form: No
Molecular Formula: C₂₆H₃₀F₃N₅O₁₀
Molecular Weight: 629.54
Cell-Permeable?: No
Purity: >90% by HPLC
Solubilities: N/A
Handling: Protect from light and moisture
Country of Origin: USA
Tag Line: A caspase-12 substrate
MDL Number: N/A
PubChem CID: N/A
SMILES: N/A
InChi: N/A
InChi Key: N/A