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Product Description
Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence VDVAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate VDVAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.
VDVAD-AFC is a ready-to-use fluorometric substrate for caspase-2 that recognize the amino acid sequence VDVAD.
Biovision | 1071 | Caspase-2 Substrate VDVAD-AFC DataSheet
Alternate Name/Synonyms: Ac-VDVAD-AFC, Caspase-2 Substrate, Fluorogenic, VDVAD-AFC, caspase2 substrate, caspase 2 substrate
Appearance: Liquid
Formulation: DMSO ( 1 mM)
CAS Number: N/A
Structure Available?: No
Peptide sequence: Ac-Val-Asp-Val-Ala-Asp-AFC
Salt Form: No
Molecular Formula: C₃₃H₄₁F₃N₆O₁₂
Molecular Weight: 770.7
Cell-Permeable?: No
Purity: >98% by HPLC
Solubilities: N/A
Handling: Protect from light and moisture
Country of Origin: USA
Tag Line: A caspase-2 substrate
MDL Number: MFCD04037014
PubChem CID: 44135100
SMILES: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(C(C)C)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
InChi: InChI=1S/C33H41F3N6O12/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17/h7-10,13-15,20-21,26-27H, 11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47)
InChi Key: BKQMJQIAIFEZJR-UHFFFAOYSA-N
