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Product Description
Ready-to-use fluorometric substrate for caspase-6 and related caspases that recognize the amino acid sequence VEID. Caspase-6 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate VEID-AFC at Ex/Em = 400/505, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase-6 assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) for caspase assays are also available separately.
VEID-AFC is a ready-to-use fluorometric substrate for caspase-6 and related caspases that recognize the amino acid sequence VEID.
Biovision | 1069 | Caspase-6 Substrate VEID-AFC DataSheet
Alternate Name/Synonyms: Ac-VEID-AFC, Caspase-6 Substrate, Fluorogenic, VEID-AFC
Appearance: Liquid
Formulation: DMSO ( 1 mM)
CAS Number: N/A
Structure Available?: No
Peptide sequence: Ac-Val-Glu-Ile-Asp-AFC
Salt Form: No
Molecular Formula: C₃₂H₄₀F₃N₅O₁₁
Molecular Weight: 727.7
Cell-Permeable?: No
Purity: >95% by HPLC
Solubilities: N/A
Handling: Protect from light and moisture
Country of Origin: USA
Tag Line: A caspase-6 substrate
MDL Number: MFCD01318833
PubChem CID: 44135099
SMILES: CCC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(C(C)C)NC(=O)C
InChi: InChI=1S/C32H40F3N5O11/c1-6-15(4)27(40-28(47)20(9-10-23(42)43)38-30(49)26(14(2)3)36-16(5)41)31(50)39-21(13-24(44)45)29(48)37-17-7-8-18-19(32(33,34)35)12-25(46)51-22(18)11-17/h7-8,11-12,14-15,20-21,26-27H,6,9-10,13H2,1-5H3,(H,36,41)(H,37,48)(H,38,49)(H,39,50)(H,40,47)(H,42,43)(H,44,45)
InChi Key: RPBOFHGWCDGAEG-UHFFFAOYSA-N
