Product Description
DAPI is a fluorescent, cell-permeable DNA-binding dye preferentially binds to DNA rich in adenine and thymine. DAPI is useful in measuring nuclear DNA content using flow cytometry for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. DAPI can also be used to detect mycoplasma and in simple retrograde double-labelling procedures for studying axonal branching employing Evans Blue and Primulin.
DAPI is a fluorescent, cell-permeable DNA-binding dye preferentially binds to DNA rich in adenine and thymine. DAPI is useful in measuring nuclear DNA content using flow cytometry for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells.
Biovision | B1098 | DAPI DataSheet
Alternate Name/Synonyms: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; 4′,6-Diamidino-2-phenylindole dihydrochloride
Appearance: Yellow solid
Formulation: N/A
CAS Number: 28718-90-3
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: Yes
Molecular Formula: C₁₆H₁₅N₅.2HCl
Molecular Weight: 350.25
Cell-Permeable?: Yes
Purity: ≥98% by HPLC
Solubilities: DMSO (>35 mg/ml)
Handling: Protect from air and light
Country of Origin: USA
Tag Line: A useful nuclear and chromosome counterstain
MDL Number: NL5995050
PubChem CID: 160166
SMILES: C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
InChi: InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChi Key: FPNZBYLXNYPRLR-UHFFFAOYSA-N