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Product Description
Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
Biovision | 7947 | HeLa Nuclear Extract (DTT-free) DataSheet
Sort Name: HeLa Nuclear Extract
Label Name: HeLa Nuclear Extract (DTT-free)
Taglines: For western blotting and HDAC assay positive controls.
Product Highlights: • Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, and 1 mM PMSF. •Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay
Packaging: EA
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Storage Temperature -70°C
Shipping dry ice
Shelf Life 12 months
