Product Description
A water-soluble tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 438 nM. Useful in cell proliferation assays as a cell viability indicator.
WST-1 is a water-soluble tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. Useful in cell proliferation assays as a cell viability indicator.
Biovision | 2198 | WST-1 DataSheet
Alternate Name/Synonyms: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt
Appearance: Pale brown solid
Formulation: N/A
CAS Number: 150849-52-8
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: No
Molecular Formula: C₁₉H₁₁IN₅NaO₈S₂
Molecular Weight: 651.35
Cell-Permeable?: Yes
Purity: ≥97% by HPLC
Solubilities: H2O (10 mg/ml); 6.5 mg/ 10ml 50mM Tris buffer, pH 8.0
Handling: Protect from air and light
Country of Origin: USA
Tag Line: A sensitive chromogenic indicator for NADH
MDL Number: MFCD01075079
PubChem CID: 6099081
SMILES: C1=CC(=CC=C1N2N=C(N=[N+]2C3=CC=C(C=C3)I)C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-])[N+](=O)[O-].[Na+]
InChi: InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33;/h1-11H,(H-,28,29,30,31,32,33);/q;+1/p-1
InChi Key: JUJBNYBVVQSIOU-UHFFFAOYSA-M