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Product Description
Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.
Biovision | 7524 | Active Caspase-1 rat recombinant DataSheet
Biomolecule/Target: Caspase-1
Synonyms: Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.
Alternates names: Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.
Taglines: A cysteine protease involved in apoptosis
NCBI Gene ID #: 8744
NCBI Gene Symbol: TNFL9
Gene Source: Rat
Accession #: P41273
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: N/A
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for the effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.
Results: >3000 units/mg
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19.5 kDa
Concentration: N/A
Appearance: Semi-Dry
Physical form description: Semi-Dry
Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A
