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Product Description
The caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The group I caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5).
Biovision | K241 | Active Human Caspases Group I Active Human DataSheet
Biomolecule/Target: N/A
Synonyms: Active Human Caspases Group I, Active Human
Alternates names: N/A
Taglines: Set of recombinant proteases involved in apoptosis
NCBI Gene ID #: CASP1- 834 CASP4- 837 CASP5 -838
NCBI Gene Symbol: CASP1 CASP4 CASP5
Gene Source: Human
Accession #: CASP1- NP_150634.1 CASP4- P49662 CASP5- P51878
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: N/A
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of the active recombinant caspase-1,-4,-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5), respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Molecular Weight: N/A
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstitute to 1 unit/µl in PBS containing 15% glycerol.
Amino acid sequence: N/A
