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Product Description
Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics, determining substrate specificity, and as a positive control in caspase activity assays.
Biovision | K233 | Active Human Caspases Set IV DataSheet
Biomolecule/Target: N/A
Synonyms: Active Human Caspases Set IV
Alternates names: N/A
Taglines: Set of recombinant proteases involved in apoptosis
NCBI Gene ID #: CASP1- 834 CASP2- 835 CASP3- 836 CASP4- 837 CASP5- 838 CASP6- 839 CASP7- 840 CASP8- 841 CASP9- 842 CASP10- 843
NCBI Gene Symbol: CASP1 CASP2 CASP3 CASP4 CASP5 CASP6 CASP7 CASP8 CASP9 CASP10
Gene Source: Human
Accession #: CASP1- NP_150634.1 CASP2- P42575 CASP3- P42574 CASP4- P49662 CASP5- P51878 CASP6- P55212 CASP7- P55210 CASP8- Q14790 CASP9- P55211 CASP10- Q92851
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: N/A
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, WEHD-pNA for caspase-4 and 5, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8 and 10 and LEHD-pNA for caspase-9) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Molecular Weight: N/A
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized powder
Reconstitution Instructions: The active recombinant caspases can be reconstituted to 0.1-1 unit per µl in PBS or the Reaction Buffer described above (available from BioVision, Cat.# 1068-20) for longer stability. We recommend using 1 unit per assay for analyzing caspase activity.
Amino acid sequence: N/A
