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Product Description
Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.
Biovision | 4010 | Active Sonic Hedgehog SHH human recombinant DataSheet
Biomolecule/Target: Sonic Hedgehog (SHH)
Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Alternates names: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Taglines: Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
NCBI Gene ID #: 5473
NCBI Gene Symbol: NAP2
Gene Source: Human
Accession #: P02775
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: 97%
Assay #2: HPLC
Endotoxin Level: <0.01 ng/g
Activity (Specifications/test method): N/A
Biological activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0-10 ng/ml.
Results: 750ng/ml.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 7.6 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20° for future use.
Amino acid sequence: N/A
