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Product Description
Tissue transglutaminase, a 78-kDa calcium dependent enzyme (EC 2.3.2.13), is found both in the intracellular and the extracellular spaces of various types of tissues. TG2 crosslinks proteins between the -amino group of a lysine residue and the -carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). TG2 also possesses deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Intracellular TG2 is thought to play an important role in apoptosis, while extracellular TG2 has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility.
Biovision | 7700 | Active Transglutaminase 2 His-tagged human recombinant DataSheet
Biomolecule/Target: Transglutaminase 2
Synonyms: TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2
Alternates names: TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2
Taglines: His tagged Human Transglutaminase 2 from E.Coli, AOF, 90% pure, biologically active.
NCBI Gene ID #: 5473
NCBI Gene Symbol: NAP2
Gene Source: Human
Accession #: P02775
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): BioVisions Active His-tagged TG2 has been tested for its activity. It has a specific activity of >400 mU/mg. 1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1 mol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric) (BioVision Cat # K571-100).
Biological activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0-10 ng/ml.
Results: >400 mU/mg
Binding Capacity: N/A
Unit Definition: 1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1 mol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric) (BioVision Cat # K571-100).
Molecular Weight: 7.6 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized from 5 mg/ml solution in a proprietary buffer.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20° for future use.
Amino acid sequence: N/A
