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Product Description
Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. It is present in blood and in most tissues, with a high concentration in lung, inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, Aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins. Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids arranged in a single polypeptide chain, cross-linked by three disulfide bridges. BioVisions Recombinant Bovine Aprotinin is an animal-origin free protein purified by proprietary chromatographic techniques.
Biovision | 7866 | Aprotinin Active Bovine Recombinant AOF DataSheet
Biomolecule/Target: Aprotinin
Synonyms: BPTI, Basic protease inhibitor, Pancreatic trypsin inhibitor
Alternates names: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor polypeptide, Becaplermin.
Taglines: >90% Pure Active, Bovine Recombinant, Animal Origin Free, Aprotinin. Can be used for inhibition of Trypsin and trypsin like enzymes
NCBI Gene ID #: 5155
NCBI Gene Symbol: PDGFB
Gene Source: Bovine
Accession #: P01127
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): BioVisions Recombinant Bovine Aprotinin has been tested for its inhibitory activity against Trypsin and Plasmin using Trypsin Activity Colorimetric Assay Kit (K771-100) and Plasmin Inhibitor Screening Kit (Fluorometric) (K382-100). The inhibitory activity of Recombinant Bovine Aprotinin (IC50 = 10.7 nM) against Trypsin was comparable to that of native Bovine Aprotinin (IC50 = 9.6 nM). The IC50 of Recombinant Bovine Aprotinin against Plasmin was 15 nM.
Biological activity: The ED as determined by the dose-dependent stimulation of thimidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of > 1 x 10 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 24.3 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 5 mg/ml solution in PBS
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A
