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Product Description
BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. (Bilirubin + NAD (P) + = biliverdin + NAD (P) H) Recombinant BLVRA protein was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | 7827 | BLVRA human recombinant DataSheet
Biomolecule/Target: BLVRA
Synonyms: Biliverdin reductase A, BLVR, BVR, BVRA
Alternates names: Biliverdin reductase A, BLVR, BVR, BVRA
Taglines: An enzyme that converts biliverdin to bilirubin
NCBI Gene ID #: 1514
NCBI Gene Symbol: CTSL1
Gene Source: Human
Accession #: P07711
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 30 pmoles/min/µg. One unit BLVRA will transform 1.0 pmole of biliverdin to bilirubin per minute in an NADPH dependent reaction pH 8.0 at 25ºC.
Results: Specific activity is > 30 pmoles/min/µg.
Binding Capacity: N/A
Unit Definition: One unit BLVRA will transform 1.0 pmole of biliverdin to bilirubin per minute in an NADPH dependent reaction pH 8.0 at 25ºC.
Molecular Weight: 33.3 kDa (295 aa, 3-296 aa)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Reconstitution Instructions: N/A
Amino acid sequence: MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK
