Product Description
Glucose Isomerase catalyzes the reversible isomerization of glucose to fructose and that of xylose to xylulose. The enzyme is widely used in the industrial production of high-fructose corn syrup (HFCS) as well as in bioethanol fermentation from hemicellulosic hydrolysates.Recombinant Glucose Isomerase protein (S. rubiginosus), fused to His-tag at N-terminus, was expressed in E.coli and purified using propriety chromatography techniques.
Biovision | P1307 | D-Glucose Isomerase Recombinant DataSheet
Biomolecule/Target: D-Glucose Isomerase
Synonyms: D-Xylose Isomerase, fructose-isomerase, D-xylose aldose-ketose-isomerase, D-xylose ketoisomerase, D-xylose ketol-isomerase
Alternates names: apm-1, Adipolean , 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, ADIPOQ, ACDC, ACRP30, APM1, GBP28
Taglines: Involved in D-xylose catabolism
NCBI Gene ID #: 11450
NCBI Gene Symbol: ADIPOQ
Gene Source: Streptomyces rubiginosus
Accession #: Q60994
Recombinant: Yes
Source: E.coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to convert 1.0 µmol of Fructose to Glucose per min. at pH 8.0 at 37 °C.
Biological activity: Determined by its ability to inhibit the proliferation of murine myeloid cell lines M1. The ED for this effect is less than 15 µg/ml.
Results: > 5 mU/mg
Binding Capacity: N/A
Unit Definition: One unit of Glucose Isomerase is the amount of enzyme that converts 1.0 µmol of Fructose to Glucose per min. at pH 8.0 at 37 °C.
Molecular Weight: 16.6 kDa
Concentration: N/A
Appearance: Ammonium sulfate suspension
Physical form description: In 50 mM Tris-HCl buffer (pH 7.0) containing 30% (NH4)2SO4 and 1 mM MgCl2
Reconstitution Instructions: Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This stock solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A