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Product Description
D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | P1050 | E. coli Recombinant IDNK DataSheet
Biomolecule/Target: idnK
Synonyms: ECK4261, gntV, JW4225
Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a
Taglines: Protein involved in the pathway L-idonate degradation, which is part of Carbohydrate acid metabolism.
NCBI Gene ID #: 3440
NCBI Gene Symbol: IFNA2
Gene Source: E. coli
Accession #: P01563
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1 x 10 IU/mg
Results: > 80 units/mg
Binding Capacity: N/A
Unit Definition: One unit is the amount of enzyme that will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
Molecular Weight: 19.4 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A
