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Product Description
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
Biovision | 6366 | E. Coli Recombinant SlyD DataSheet
Biomolecule/Target: N/A
Synonyms: E. Coli Recombinant SlyD
Alternates names: FKBP-type peptidyl-prolyl cis-trans isomerase.
Taglines: Exhibits several activities including that of a peptidyl-prolyl isomerase
NCBI Gene ID #: Rn. 6075
NCBI Gene Symbol: EGF
Gene Source: E. Coli
Accession #: P07522
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is >220 nmoles/min/mg.
Results: >220 nmoles/min/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that cleaves 1 µmole of Suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.
Molecular Weight: 21 kDa (196 aa, 1-196 aa)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl (pH 7.5).
Reconstitution Instructions: N/A
Amino acid sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
