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Product Description
Polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues. Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences. The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein. Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAca1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen. GalNAc-T2 is primarily located in Golgi, and is widely distributed in human tissues.
Biovision | P1215 | GalNAc-T2 soluble fragment Human Recombinant DataSheet
Biomolecule/Target: GALNT2
Synonyms: Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, GaNTase 2
Alternates names: Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, GaNTase 2
Taglines: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis
NCBI Gene ID #: 2254
NCBI Gene Symbol: FGF9
Gene Source: Human
Accession #: P54130
Recombinant: Yes
Source: Insect cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by transfer of GalNAc from UDP-GalNAc to the peptide MEELGMAPALQPTQGAMPAF.
Biological activity: Specific activity is greater than 300 pmol/min/µg. Measured by transfer of GalNAc from UDP-GalNAc to the peptide MEELGMAPALQPTQGAMPAF, enzyme reactions containing: 25 mM Calcium carbonate pH 7.4, 10 mM manganese chloride, 0.25% Triton X 100, 2 mM UDP-GalNAc and 10 µg (400 µg/mL final) peptide. Incubated at 37 ºC.
Results: >300 pmol/min/ug
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: N/A
Concentration: 360 ug/ml
Appearance: Liquid
Physical form description: Sterile filtered solution in 20 mM Hepes pH 7 and 100 mM NaCl
Reconstitution Instructions: N/A
Amino acid sequence: N/A
