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Product Description
Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Biovision | 7808 | GOT1 human recombinant DataSheet
Biomolecule/Target: GOT1
Synonyms: Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18
Alternates names: Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18
Taglines: Plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
NCBI Gene ID #: 27006
NCBI Gene Symbol: FGF22
Gene Source: Human
Accession #: Q9HCT0
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 13 unit/mg, in which one unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
Results: Specific activity is > 13 unit/mg
Binding Capacity: N/A
Unit Definition: One unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
Molecular Weight: 48.4 kDa (433 aa, 1-413 aa + His Tag)
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 100 mM NaCl and 1 mM DTT.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
