Product Description
Recombinant Human Heat Shock Protein produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids and have a molecular weight of 22.7 kDa. The protein was purified by proprietary chromatographic techniques.
Biovision | 4853 | Heat Shock Protein 27 human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: N/A
Alternates names: FS, activin-binding protein, FSH-suppressing protein (FSP)
Taglines: A chaperone protein that assists protein folding
NCBI Gene ID #: 14313
NCBI Gene Symbol: FST
Gene Source: Human
Accession #: P47931
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 90%
Assay: SDS-PAGE
Purity: 98%
Assay #2: HPLC
Endotoxin Level: <0.1 ng/g
Activity (Specifications/test method): N/A
Biological activity: The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.
Results: 0.3 ug/mL
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 31.6 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: 1 mg/ml solution containing 20 mM Hepes (pH 7.5), 1 mM DTT and 100 mM KCl.
Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Amino acid sequence: MGNCWLRQAK NGRCQVLTKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DS