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Product Description
CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, 51 integrin and IIb3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
Biovision | P1175 | Human CellExp CD40 Ligand / TNFSF5 Fc tag Human Recombinant DataSheet
Biomolecule/Target: CD40LG
Synonyms: CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39
Alternates names: Erythropoietin-, EPO-, Epoetin, EP, MGC138142
Taglines: Costimulates T-cell proliferation and cytokine production
NCBI Gene ID #: 2056
NCBI Gene Symbol: Epo
Gene Source: Human
Accession #: P01588
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per/g
Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.
Biological activity: Determined by a cell proliferation assay using TF-1 cells. The expected ED50 for this effect is 0.8-1.0 ng/ml.
Results: N/A
Binding Capacity: Immobilized Human CD40 Ligand, His Tag at 5 g/mL (100 l/well) can bind Human CD40, Fc Tag with a linear range of 0.156-5 g/ml.
Unit Definition: N/A
Molecular Weight: 37 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 m filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers containing a carrier protein (like 0.1% BSA) and stored in working aliquots at -20°C to -80ºC for future use.
Amino acid sequence: N/A
