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Product Description
The cytokine IL-10 is a key anti-inflammatory mediator with an essential role in the development and proliferation of regulatory T cells, restricting myeloid and chronic inflammatory T cell responses. It has been shown that in solution IL-10 predominantly exists as a homodimer. However, the subunits within the dimer are non-covalently associated, although each subunit contains two intra-chain disulfide bonds. IL10 binds to its hetero-tetrameric receptor comprising IL10RA and IL10RB, which activates multiple pathways that contribute to the downstream anti-inflammatory effects. It also plays important roles in other processes such as sterile wound healing, autoimmunity, cancer, and homeostasis. Given the wide spectrum of the anti-inflammatory properties attributed to IL-10, both IL-10 enhancing and blockade strategies have been tried in a number of diseases and cancer therapy.
Biovision | 6466 | Human CellExp IL-10 Human Recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Alternates names: Thymic Stromal Lymphopoietin
Taglines: An anti-inflammatory cytokine
NCBI Gene ID #: 85480
NCBI Gene Symbol: TSLP
Gene Source: Human
Accession #: Q969D9
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 g of protein
Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using BioVisions Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.
Biological activity: The ED determined by the ability to induce proliferation of human Interleukin-7 receptor & human TSLP Receptor co-transfected with mouse BaF3 cells was found to be less than 0.3 ng/ml.
Results: <0.3 ng/ml.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 15.0 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized
Reconstitution Instructions: Reconstitute in sterile dHO to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A
