Product Description
Kallikrein-8 (KLK8) is also known as Neuropsin (NP or NRPN), Ovasin, Serine protease 19 (PRSS19), Tumor-associated differentially expressed gene 14 protein (TADG-14), which belongs to the peptidase S1 family and Kallikrein subfamily. KLK8 contains 1 peptidase S1 domain. KLK8 is pH dependence protein and the optimum pH is 8.5, and the protein is active from pH 7-10. KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. KLK8 cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys, and the catalytic activity of KLK8 is inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.
Biovision | 7511 | Human CellExp KLK-8 / Kallikrein-8 human recombinant DataSheet
Biomolecule/Target: KLK-8 / Kallikrein-8
Synonyms: KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
Alternates names: KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
Taglines: 95% pure, active, human cell expressed, human recombinant KLK-8 / Kallikrein-8 - serves as a marker of ovarian cancer.
NCBI Gene ID #: 3553
NCBI Gene Symbol: IL2
Gene Source: Human
Accession #: P60568
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/g by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >500 pmoles / min / g.
Results: >500 pmoles / min / g
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 25.8 kDa. The predicted N-terminus is Gln 29. DTT-reduced Protein migrates as 38 kDa in SDS-PAGE due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Amino acid sequence: Gln 29 Gly 260