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Product Description
Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.
Biovision | 7513 | Human CellExp Osteoactivin / GPNMB human recombinant DataSheet
Biomolecule/Target: Osteoactivin / GPNMB
Synonyms: GPNMB, HGFIN, NMB, Osteoactivin
Alternates names: GPNMB, HGFIN, NMB, Osteoactivin
Taglines: 95% pure, active, human cell expressed, human recombinant Osteoactivin / GPNMB - acts as a downstream mediator of BMP-2.
NCBI Gene ID #: 124334
NCBI Gene Symbol: IL3
Gene Source: Human
Accession #: P04823
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/g by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 4 - 16 µg/ml.
Results: ED50 for this effect is typically 4 - 16 µg/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 52.9 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 85-100 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Amino acid sequence: N/A
