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Product Description
Tissue factor pathway inhibitor (TFPI) also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a Nterminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a Cterminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The Cterminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
Biovision | 7453 | Human CellExp TFPI human recombinant DataSheet
Biomolecule/Target: TFPI
Synonyms: TFPI, LACI, TFPI1, EPI, TFI
Alternates names: TFPI, LACI, TFPI1, EPI, TFI
Taglines: A protein which reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa)
NCBI Gene ID #: 16193
NCBI Gene Symbol: IL6
Gene Source: Human
Accession #: P08505
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/g by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval WRK (Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 l reaction mixture containing 1.25 ng trypsin, 10 M substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5.
Results: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval WRK (Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 l reaction mixture containing 1.25 ng trypsin, 10 M substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 30 kDa. The predicted N-terminus is Asp 29. DTT-reduced Protein migrates as 41-45 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Amino acid sequence: N/A
